Silica gel-based reverse-phase or normal-phase columns for solid-phase extraction. In both cases, explosives can be eluted from the SPE cartridge already dissolved in reversed-phase HPLC-compatible solvents, greatly simplifying sample transfer. Description. Solid Phase Extraction . . You can optimize your SPE protocols by using SiliCycle SiliaPrepX Polymeric SPE Cartridges and Well Plates.. AttractSPEHLB are uncharged water-wettable polymeric resin suitable for the extraction of a wide range of analytes (polar, apolar, neutral, acidic, basic). 4-Step SPE Procedure. Solid-phase extraction (SPE) is a sample preparation method where the compounds that are dissolved or suspended in a liquid mixture are separated from other compounds depending on their physical and chemical properties. Excellent recovery rates, especially for the enrichment of pharmaceuticals and active ingredients, due to the spherical particle shape, homogeneous surface, and optimized pore structure. Features of Reversed Phase C18 Solid Phase Extraction (SPE) Cartridges These products are available in more than 200 chemistry and format combinations for convenient sample preparation methods based on reverse-phase, normal-phase, ion-exchange, and application-specific specialty chemistries. Solid phase extractions (SPEs) are routinely used in the sample preparation for quantifying analytes in biological fluids such as plasma and urine. Copure C18N SPE Cartridges 2000mg/12mL 20/Box Write a Comment. Use Thermo Scientific SOLA Solid Phase Extraction (SPE) cartridges and plates to help prevent costly reanalysis of bioanalytical samples by preventing blocking, voiding, and channeling during the SPE sample preparation process. Reverse Phase. N/A. External dry loading using reversed-phase media as the dry load sorbent is a very efficient way to protect your reversed-phase flash cartridge from contamination. This first fritless SPE product range enables greater sample throughput in bioanalytical laboratories where low elution volumes combined with greater . We called this reversed phase SPE. SPE makes use of a solid phase material (there are many to choose from) that . In other words, it may be difficult for a reversed-phase method or bonded chemistry to differentiate between molecules that are structurally similar. N/A. Filled with stationary phase compositions . From Email Email notification Text Comment will be posted once reviewed. . Activation: Rinse the extraction cartridge with 3-5 ml methanol; rinse the extraction cartridge with 3-5 ml deionized water (or buffer) and keep the extraction cartridge moist 2. Suzuki Catalysts Scavenging Study with Activated Carbon and SiliaMetS; Amine sorbent for the separation of polar compounds; Using Silica-Based Products for Food and Drug Applications; Plastic Chemical Resistance Guide; Reversed-Phase Chromatography: General Introduction for Improved Method Development Details. The type of SPE sorbent is chosen based on the chemical properties of the species we wish to separate. The hydrophobic reverse phase filler can retain most of the non-polar compounds and most of the organic analytes in the aqueous matrix. Reversed-phase SPE was used in a novel online SPE-HPLC electrochemical and UV detection system for field analysis, with equivalent goals to US EPA Method 8330. Solid Phase Extraction. spe columnspe cartridgesolid phase extraction cartridgesolid phase extraction column. For reverse phase, normal phase and adsorption type SPE cartridges, the quantity of target analytes to SPE sorbent . Solid Phase Extraction. Polymeric MCX SPE cartridges sorbent has a larger surface area compared with other sorbents, so it has a high ion exchange capacity which can keep alkaline material effectively Skip to content +86-29-89284429 Restek. High-purity material with highest reproducibility and lowest blank values due to an optimized manufacturing process. AttractSPE DVB is available under Disk and Powder format as well as under several SPE cartridges size from 1mL to 20mL , reversible formats and for automate. [SPE Column Silica gel-based Normal / Reverse Phase] .We manufacture and distribute chemical reagents for research use only or various antibodies. The analyte can be eluted by washing the cartridge with a non-polar solvent, which disrupts the interaction of the analyte and the stationary phase. Activated carbon; 50 micron irregular (ISOLUTE-NH2) The technique offers targeted selectivity for analytes of interest, versatility for a wide range of sample volumes, can be easily automated for high-throughput, and is performed by following quick and simple methods (condition, equilibrate, load, wash, elute). The Agilent SampliQ WAX solid-phase extraction cartridges are compatible with water, acid, basic solvents from pH 0 to 14, and most organic solvents. 1. The first step in using SPE is to select the correct type of adsorbent for the type of separation that is needed. Reversed-Phase SPE; Normal-Phase SPE; Ion-Exchange SPE; Copure Adsorption SPE; Copure Mixed SPE; Copure Specialty SPE; Copure MS SPE; Copure SLE; Copure PPT; SPE Vacuum Manifolds; Life Science. SMT TotalSep SPE cartridges have been developed to meet some of the . C18 silica is end-capped. Resprep S-DVB SPE Cartridge. $112.20 - $339.50 See promotional offers below Specifications View More Specs Products 15 Description Specifications Description . Solid-Phase Extraction (SPE) Columns and Cartridges . Solid phase extraction (SPE) is a sample preparation technique often used by chromatographers prior to analysis. C18, SPE Cartridges, Oro-Sep, 300.0 mg, 100/pkg Reversed phase retention mechanism of the C18 elute allows for the extraction of hydrophobic compounds from aqueous matrices. Copure C18 sorbent is composed of endcapped octadecyl-bounded silica gel particles and retains non-polar compounds by hydrophobic interactions. Especially suited for convenient solid phase extraction of small sample volumes. SPE phase selection overview. Life sciences & Pharmaceutical: drug discovery, pre-clinical and clinical development, toxicology studies, medical . exchange/reversed phased) behavior provides greater flexibility in method design, the ability to fractionate neutral compounds, and cleaner extracts. 3 mL, 500 mg. 500 mg/3 mL. Copure QuEChERS . Reversed-Phase SPE; Copure C18N SPE Cartridges; . Apply sample solution to the top of the packing bed. Details. Choosing the best SPE stationary phase to use depends on the physical/chemical properties (such as pH, pKa and Solubility log P) of your analytes. Excellent recovery rates, especially for the enrichment of pharmaceuticals and active ingredients, due to the spherical particle shape, homogeneous surface, and optimized pore structure. Typical applications include . 22, 23 and 30) Reverse SPE tubes . However, such blended SPE products do exist, allowing you to utilize ion-exchange and reversed-phase retention mechanisms in the same SPE medium (e.g., polymeric ion-exchange SPE sorbents). Sorbent Charge Can be Activated or Deactivated. Reversed-Phase SPE Sorbent surface is relatively hydrophobic -Sep-Pak C18 Silica, other alkyl silica, Oasis HLB Sample is prepared for SPE in aqueous or other polar sorbent Oasis HLB is better -pH stable from 1-14 -Doesn't de-wet Examples: Isolation and enrichment of organic contaminants from surface waters Highly reproducible recovery rates; cartridge to cartridge, lot to lot. Wide range of over 25 adsorbents to meet any application need. Do not let packing dry. Complementary Reversed Phase Retention of Neutral Compounds. Thermo Scientific HyperSep solid phase extraction cartridges offer reproducible, reliable sample preparation in a traditional format. An important distinction to keep in mind when employing ion-exchange mechanisms is the nature of the charge state of the analyte. Ion Exchange. Award-winning fritless polymeric technology eliminates the issues encountered in conventional SPE so that your lab can: Applications using C18 silica SPE cartridges are found in the pharmaceutical, environmental, and forensic sci-ence industries. The analyte can be eluted by washing the cartridge with a non-polar solvent, which disrupts the interaction of the analyte and the stationary phase. The C18 solid phase extraction (SPE) cartridge uses octadecyl silica as a filler to retain non-polar compounds by strong hydrophobic interaction. SOLA WAX SPE Cartridge: Bed Weight: 10 mg: For Use With (Application) Bioanalytical Applications - Strong Ion-Exchange Retention of Strong Acidic Compounds. Solvate the silica-bonded phase or polymer packing with six to ten hold-up volumes of methanol or acetonitrile. The hydrophobic reversed phase material is retentive for most nonpolar compounds, and retains most organic analytes from aqueous matrices. . Pore Size: 40 - 450 . Copure C18A SPE Cartridges 100mg/1mL 100/Box COC18A3200 . Compared to liquid-liquid extraction, SPE is much faster, uses less solvent, and saves money. The force between non-polar substances is van der Waals attraction, and the affinity of the agent to the solute mainly depends on its hydrophobicity. The stationary phase of a reversed phase SPE cartridge is derivatized with hydrocarbon chains, which retain compounds of mid to low polarity due to the hydrophobic effect. Reversed phase (C18, C8, cyano, phenyl) Normal phase (silica, diol, NH 2) Ion . [3] The precision and accuracy of the The cartridge . Product Category. Three prominent SPE procedures are normal phase (Polar) SPE, reversed phase (Non-Polar) SPE, and Ion exchange SPE. Rinse packing Bed with 3-5 mL methanol. Compared with C18, C8 has shorter carbon chains and moderate hydrophobicity, thus makes an alternative for extracting compounds that is strongly retained by C18 sorbent. An important distinction to keep in mind when employing ion-exchange mechanisms is the nature of the charge state of the analyte. However, because reversed-phase will retain most molecules with any . This item has been discontinued by the manufacturer and is no longer available. Solid phase extraction (SPE) is a technique designed for rapid, selective sample preparation and purification prior to the chromatographic analysis (e.g. The full range of solid phase extraction (SPE) well plates, columns, disks and accessories from Biotage. CHROMAFIX cartridges offer an alternative way of handling using positive pressure by syringes or peristaltic pumps.. Sample Prep. Oasis HLB cartridges contain the Oasis HLB sorbent, which is a universal polymeric reversed phase sorbent that was developed for the extraction of a wide range of acidic, basic, and neutral compounds from various matrices using a simple, generic protocol.Chromatography columns separate molecules within mixtures during gas or liquid chromatography. C18 reverse phase silica (11%, 17% or 23% carbon content; 60 ; mesh 230 - 400) Amine . The hydrophobic reversed phase material is retentive for most nonpolar compounds, and retains most organic analytes from aqueous matrices. Product Overview Videos Documents Recommendations Showing 10 of 14 Show more Reversed Phase C18 SPE Cartridges Hawach Reversed Phase C18 SPE Cartridges have a high retention rate of the alkyl bonding phase for non-polar to moderate polar compounds. Resprep S-DVB SPE Cartridge High-purity material with highest reproducibility and lowest blank values due to an optimized manufacturing process. Solid-Phase Extraction (SPE) is a sample preparation technique that is used to clean up and/or concentrate samples before analysis. Reversed Phase Cartridges. C18, SPE Cartridges, Oro-Sep, 300.0 mg, 100/pkg Reversed phase retention mechanism of the C18 elute allows for the extraction of hydrophobic compounds from aqueous matrices. Catalog number: 60109-001. Technical Support Customer Service. Features of Chromabond and Chromafix SPE Portfolio. AttractSPESAX is SPE based on strong anion exchange polymeric phase interacting with the analytes via a mixed mode retention mechanism: ion exchange with strong basic functional groups and reversed phase. Used to extract hydrophobic analytes from polar matrices, such as water (e.g., pesticides from water). HyperSep SPE Cartridges and Plates. A SPE cartridge consists of a small, open-ended container (often a syringe barrel) filled with particles coated with a sorbent. Solid-phase extraction (SPE) is a technique designed for rapid sample preparation and purification prior to chromatographic analysis. High-quality, silica-based hydrophobic adsorbents. Solid phase extraction (SPE) is an effective technique for cleaning up and concentrating samples, prior to HPLC or GC analysis. 4. This is the most commonly used sorbent for sample preparation prior HPLC or GC analysis. . 50/Pk. Some of these sorbents are the same types used in HPLC columns. Description. Female Luer fitting at the inlet, male Luer tip as exit. It can retain most organic compounds and is widely used in areas such as environmental monitoring and food safety. . Collect sample for analysis if desired compound has passed through the bed without being retained. This item has been discontinued by the manufacturer and is no longer available. Particle Size: 5 m - 250 m. We mainly offer three basic packing types for normal phase, reverse phase and ion exchange extraction as follows: Silica-based C18, C18-ne, C8, C2, PHE, CN, PSA, NH2, SAX, SCX, Silica Polymer-based . HPLC, GC, TLC). The assay involved a solid-phase extraction (SPE) of olanzapine and its internal standard on a Bond Elut Certify LRC mixed-mode cartridge. A general protocol can be used to separate weakly basic, weakly acidic, and neutral compounds from complex mixtures. Common in environmental Used for large scale purication Reverse SPE tubes prior to elution analysis to reduce leachables (medicinal chemistry purication) to minimize elution volume from PP hardware and PE frits for strongly retained compounds Supel-Tips SPE Rezorian Cartridges Discovery SPE 96-Well Plates Dispersive SPE HLB SPE cartridges. Solid phase extraction (SPE) is a technique designed for rapid, selective sample preparation and purification prior to the chromatographic analysis (e.g. Solid phase extraction (SPE) is a much faster purification method if optimized and it can be done with the standard cartridges as well as 96-well plates, that allow many samples to efficiently be run at the same time. Thermo Scientific HyperSep C18 Cartridges Get improved retention for most nonpolar compounds and organic analytes in aqueous matrices with these hydrophobic reversed phase C18 cartridges. . Reversed-Phase SPE For extracting non-polar compounds from complex matrices, Biocomma offers several reversed-phase SPE cartridges: most commonly used C18 (endcapped and non-endcapped) and C8. Product Line: SOLA: Sorbent: Mixed Mode Weak Anion Exchanger, Polymeric: Type . Flush the cartridge with aqueous solvent to remove pH modifiers and buffers. Solid-phase extraction is designed for rapid sample preparation and purification prior to chromatographic analysis. The C8 SPE cartridge is a moderately hydrophobic, reverse phase silica matrix filler that retains non-polar compounds through hydrophobic interactions. Steroids Extraction By Reversed-Phase Chromatography; SPE Extraction Of Oil & Grease From Water Using SiliaPrep C18 (17%) SPE Extraction Of Phenolic Compounds From Wine Using SiliaPrep C18 (17%) SPE Extraction Of Streptomycin From Food Using SiliaPrep SCX And C18 (17%) Normal Phase. Restek. Save Big with Phenomenex's Legendary Savings http://www.phenomenex.com/info/form/17yearendpromoA quick look at how Strata-X polymeric sorbents can simplify t. In . Copure C8 sorbent is composed of octyl-bounded silica gel particles and retains non-polar compounds by hydrophobic interactions. A novel 2-layer SPE cartridge efficiently reduces background for pesticide determination. Step 1: Conditioning. Reversed-phase SPE is considered the least selective retention mechanism when compared to normalphase or ion-exchange SPE. The stationary phase of a reversed phase SPE cartridge retain compounds of low polarity due to the hydrophobic effect. 30 micron spherical (SFAR C18) 50 micron irregular (KP-C18-HS) Speciality Phase Cartridges. Introduction of Three SPE Cartridges' Operation Methods Operation of the reverse phase extraction cartridge Extract non-polar samples from polar matrices 1. 28 Online SPE-HPLC was accomplished using a combination of two six-port valves. SPE provides clean extracts and high recoveries. 24050. The analyte is removed from the adsorbent by elution with a suitable solvent. . Solid phase extraction (SPE) is a variant of liquid chromatography. Polar extraction is where the stationary bed is strongly polar in nature and the mobile phase is non-polar or . To perform reversed-phase chromatography with SPE cartridges, use a gradient of strongly to weakly polar solvents [from weak to strong solvent elution strength] with a non-polar packing material. Steroids Extraction By Reversed-Phase Chromatography; SPE Extraction Of Oil & Grease From Water Using SiliaPrep C18 (17%) SPE Extraction Of Phenolic Compounds From Wine Using SiliaPrep C18 (17%) SPE Extraction Of Streptomycin From Food Using SiliaPrep SCX And C18 (17%) Grow your laboratory productivity. Solid Phase Extraction (SPE) Cartridges & Well Plates . Solid phase extractions use the same type of stationary phases as are used in liquid chromatography columns. SPE is most often used to remove interfering compounds from a sample, although it can also be used to enrich/concentrate analytes of interest in the sample. PREVIOUS Glass Bottom Plates. We offer a range of solid phase extraction (SPE) columns and cartridges, packed with a variety of sorbents including regular silica gel 60 and reverse phase silica gels (endcapped and non-endcapped). One clear advantage in SPE-HPLC of explosives, as demonstrated in this and the previous reference, is the . Always store reversed-phase cartridges in your method's strong solvent (without pH modifiers or . However, such blended SPE products do exist, allowing you to utilize ion-exchange and reversed-phase retention mechanisms in the same SPE medium (e.g., polymeric ion-exchange SPE sorbents). All Sep-Pak Cartridges contain the high quality sorbents and chemistries that provide highly reproducible SPE results. Stable over the pH range (2 - 8). Description Reversed Phase C18A SPE Cartridges The C18A SPE cartridge uses octadecyl silica as a filler to extract non-polar compounds from an aqueous solution by hydrophobic interaction. The filler retains most of the organic matter and is widely used in the fields of environmental and food safety. After conditioning of the SPE cartridge, human milk (1 ml . Reversed-Phase SPE Time Hot Copure C18N SPE Cartridges Copure C18A SPE Cartridges Copure C18 SPE Cartridges Copure C8 SPE Cartridges 1/1 Using SiliaPrepX will guarantee the following:. Types. General Extraction Protocols: Reversed Phase Rinse packing bed with 3 - 5 mL methanol. Resprep C18 SPE Cartridges. HPLC, GC, TLC). Remove unwanted contaminants including phospholipids, proteins, and particulates for superior chromatography Easily cleanup large or small volumes Strata-XL cleans up viscous samples as well as target analytes that exceed 10 kDa The 96-well plate microelution Strata-X formats are ideal for concentrated extraction for small volumes Order Now SPE is most often used to remove interfering compounds from a sample, although it can also be used to enrich/concentrate analytes of interest in the sample. Reversed phase non-polar (hydrophobic) SPE . The present study reports a precise and simple offline solid-phase extraction (SPE) coupled with reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous determination of five representative and commonly present pharmaceuticals and personal care products (PPCPs), a new class of emerging pollutants in the aquatic environment. Reversed phase mode refers to the separation mode in which the polarity of the stationary phase of the SPE cartridge is less than the polarity of the mobile phase. In SPE, one or more analytes from a liquid sample are isolated by extracting, partitioning, and/or adsorbing onto a solid stationary phase. Reversed Phase SPE . 4-Step Method. 50/Pk. The bonded octadecyl functional group allows for increased binding capacities with higher recoveries. The bonded octadecyl functional group allows for increased binding capacities with higher recoveries. 24050. Rinse with 3 - 5 mL water or buffer. Certificates of analysis for this product are provided electronically. Thermo Scientific SOLA SPE Cartridges revolutionize solid phase extraction (SPE). Reversed phase SPE separates analytes based on their polarity. Compared with the C18 filler, the C8 filler has a shorter carbon chain and a weaker non-polar hydrophobic interaction. With reversed phase (RP) SPE, the dimethoxytrityl (DMT) group, that is attached to the This is the most commonly used sorbent for sample preparation prior HPLC or GC analysis. Thermo Scientific HyperSep C18 Cartridges feature a highly retentive alkyl-bonded phase for nonpolar to moderately polar compounds. Available in silica and polymer-based phases for reverse phase, ion exchange, and specific-application needs. Push or draw through the bed at 1 - 5 mL/min. Column, well plate, and cartridge formats. SPE cartridges are available with many different sorbents. Product Code Product Name Filler Content (mg/cartridge) Package Size; 297-47451: Solid Phase Extraction (SPE) Application Notes. Please call customer service for assistance: 1-800-766-7000. There are a variety of SPE stationary phases to choose from including: reverse phase, normal phase, ion-exchange, and mixed mode, with mixed mode being a mixture of both . 2: Rinse packing bed with 3-5 mL water or buffer. C18 Solid Phase Extraction Spe Cartridge , Find Complete Details about C18 Solid Phase Extraction Spe Cartridge,Solid Phase Extraction,Spe Cartridge,200mg Spe Column from Supplier or Manufacturer-Hawach Scientific Co., Ltd. . Agilent SampliQ C18 is a reversed-phase sorbent. . The following specifications of SPE cartridges are available: Volume: 0.01 ml - 200 ml. Reversed-phase SPE is considered the least selective retention mechanism when compared to normalphase or ion-exchange SPE. QuEChERS AOAC Kits; QuEChERS EN Kits . Typical applications include . Chromatographers use solid phase extraction (SPE) as a sample preparation method-of-choice to improve analytical results by removing interfering compounds, extracting the analyte in a preferred solvent, and concentrating the analyte. Perform solid phase extraction (SPE) using columns and 96-well plates for sample preparation that improves chromatographic results. Chemistries. Share sensitive information only on official, secure websites. 5. Please call customer service for assistance: 1-800-766-7000. In other words, it may be difficult for a reversed-phase method or bonded chemistry to differentiate between molecules that are structurally similar. A locked padlock) or https:// means you've safely connected to the .gov website. It is particularly suited for the extraction of weak acids (pKa 2 - 8). High quality products covering a broad spectrum of applications for a wide range of complexe matrices. . Reversed-phase SPE cartridges use non-polar adsorbents (carbohydrates or polymers) on the stationary phase to separate organic solutes from the polar phase (water). SPE cartridges. 3 mL, 500 mg. 500 mg/3 mL. Solid Phase Extraction Products SPE Tubes and Specialty Hardware Quick Look-Up Guide Additional Tubes and SPE Configurations Custom Capabilities Glass SPE Tubes with PTFE and SS Frits (pg. These correspond to the usual filling materials of HPLC columns, such as reversed phase, normal phase, ion exchange or mixed-mode phase. Solid phase extraction (SPE) is a sample preparation technique often used by chromatographers prior to analysis. 29 and 30) Common in environmental analysis to reduce leachables from PP hardware and PE frits Reversible SPE Tubes (pg. The solid phase is simply used to "filter" the sample. Solid Phase Extraction .
reverse phase spe cartridge
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